Cytotoxic T cell adjuvant effects of three Salmonella enterica flagellins
نویسندگان
چکیده
Bacterial flagellins are important virulence-associated factors and strong inducers of inflammatory responses in mammalian hosts. Flagellins have also been investigated as potential vaccine adjuvants, either for induction of humoral or cellular immune responses, to different target antigens. In this study we investigated the adjuvant properties of three Salmonella enterica flagellins types (FliCd, FliCi and FljB) to an ovalbumin-derived CD8(+) T cell-restricted epitope (OVA257-264). Although mice immunized with the three tested flagellins elicited antigen-specific activated CD8(+) T cells, only animals immunized with FliCi and FliCd flagellins admixed with ovalbumin mounted specific in vivo cytotoxic responses to peptide-pulsed target cells. The present results indicate that Salmonella flagellins are endowed with type-specific adjuvant effects toward murine CD8(+) T cells, a feature that may impact their use as adjuvants for prophylatic or therapeutic vaccines.
منابع مشابه
Host and bacterial factors affecting induction of immune responses to flagellin expressed by attenuated Salmonella vaccine strains.
Previous observations demonstrated that the delivery of recombinant Salmonella enterica serovar Dublin strains to mice via mucosal routes did not efficiently activate systemic and secreted antibody responses to either type d flagellin or genetically fused heterologous B-cell epitopes, thus reducing the usefulness of the protein as a carrier of epitopes for vaccine purposes. In this work, we inv...
متن کاملA scalable method for biochemical purification of Salmonella flagellin
Flagellins are the main structural proteins of bacterial flagella and potent stimulators of innate and adaptive immunity in mammals. The flagellins of Salmonella are virulence factors and protective antigens, and form the basis of promising vaccines. Despite broad interest in flagellins as antigens and adjuvants in vaccine formulations, there have been few advances towards the development of sc...
متن کاملCD4+-T-cell responses generated during murine Salmonella enterica serovar Typhimurium infection are directed towards multiple epitopes within the natural antigen FliC.
The flagellar filament protein FliC is a natural antigen recognized by memory CD4+ T cells recovered from Salmonella enterica serovar Typhimurium-infected humans and mice. To further investigate T-cell responses to FliC, we derived FliC-specific CD4+-T-cell clones from mice of two different haplotypes following oral S. enterica serovar Typhimurium infection. Using C-terminal truncations of MalE...
متن کاملIn silico Studies for Comparison of FliCs from Salmonella typhimorium, Pseudomonas aeruginosa, and Escherichia coli and their Implication as an Adjuvant
An increasing number of studies have demonstrated the effectiveness of flagellin (FliC) as an adjuvant. In this study, FliC(s) from Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa, and Escherichia coli were compared. In silico studies were designed to determine physicochemical parameters of FliC from different bacteria. The fliC gene corresponding to best model was amplified, cl...
متن کاملEnhancement of Host Immune Responses by Oral Vaccination to Salmonella enterica serovar Typhimurium Harboring Both FliC and FljB Flagella
Flagellin, the structural component of the flagellar filament in various motile bacteria, can contribute to the activation of NF-κB and proinflammatory cytokine expression during the innate immune response in host cells. Thus, flagellin proteins represent a particularly attractive target for the development of vaccine candidates. In this study, we investigated the immune response by increasing ...
متن کامل